Journal: iScience

Article Title: Fabrication of RADA32/Ngf_EE/MSCs composite hydrogel and its protective mechanism against radiation-induced ototoxicity

doi: 10.1016/j.isci.2026.115723

Figure Lengend Snippet: In vivo protective effects of RNM composite gel (A) Schematic of the in vivo experimental timeline and anatomical images of the mouse cochlea under a dissection microscope. (a, oval window; b, cochlear labyrinth.). (B–G) ABR tests performed 2 days before radiation exposure and 3, 7, and 14 days after drug administration to assess hearing function in mice. (H) Left: Confocal microscopy images of the cochlear basilar membrane 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel, stained with FITC-phalloidin to label hair cells. Scale bars, 20 μm. Right: Quantitative comparison of outer hair cell (OHC) and inner hair cell (IHC) survival rates post-intervention. Data are represented as the mean ± SEM ( N = 3, t test). (I) H&E staining images of mouse cochlear tissues extracted 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel. Data are represented as the mean ± SEM ( N = 3, t test). Scale bars, 20 μm. Significant differences between the groups are indicated by ∗ for p < 0.05, ∗∗ for p < 0.01, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001.

Article Snippet: The whole-mount basilar membranes were stained with FITC-conjugated phalloidin (Catalog #G1248-100T, Servicebio) and observed under a laser scanning confocal microscope for hair cell morphology assessment and counting.

Techniques: In Vivo, Dissection, Microscopy, Confocal Microscopy, Membrane, Injection, Staining, Comparison

Journal: Molecular Therapy Advances

Article Title: Topical delivery of a PPARγ agonist enables adipogenic repair of irradiated skin

doi: 10.1016/j.omta.2026.201732

Figure Lengend Snippet: Topical rosiglitazone enhances wound healing in irradiated skin (A) Schematic of in vivo radiation and wound healing design. (B) Representative images of wound healing progression in irradiated mice treated with TC (top) or TR (bottom). (C) Kaplan-Meier curve showing time to complete wound closure in TC- and TR-treated irradiated mice. (D) Representative immunofluorescence (IF) images of CD31 expression (20×; scale bars, 100 μm). (E) Quantification of CD31 IF intensity (arbitrary units, a.u.). (F) Representative Picrosirius red-stained images under polarized light, showing collagen fiber maturity and organization (20×; scale bars, 100μm). (G) Quantification of collagen composition (percent area) for type 3 (green/yellow) and type 1 (orange/red) collagen. n = 7 mice/group for wound healing analyses; n = 6–7 mice/group for IF and histology. For IF and Picrosirius: one section/mouse, four high-power fields (hpf)/section (4 measurements/mouse). Statistical analysis: log-rank test (C), unpaired Student’s t test (E, G). ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: For IF imaging using fluorescein isothiocyanate (FITC)-conjugated CD31, cryosectioned tissues were incubated overnight at a concentration of 1:100 (Invitrogen, catalog #2373735).

Techniques: Irradiation, In Vivo, Immunofluorescence, Expressing, Staining